Inhaltsbereich

• Sample preparation for IHC by perfusion of rats and mice with fixative.
• Sectioning of tissue for IHC by cryomicrotomy.
• Detection of cells, receptors, neuronal transmitters, phosphorylation sites etc. by application of specific antibodies and visualization by fluorescence microscopy.

• Sample preparation for Western Blot by various extraction techniques.
• Separation of proteins by SDS-PAGE electrophoresis, followed by blotting onto nitrocellulose membrane.
• Chemiluminescent detection of specific proteins and/ or their  phosphorylated form.

• DNA-isolation from laboratory mice tissue for molecular analysis.
• Identification and amplification of DNA fragments by PCR in order to verify gene expression in transgenic mice.