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- Sample preparation for IHC by perfusion of rats and mice with fixative.
- Sectioning of tissue for IHC by cryomicrotomy.
- Detection of cells, receptors, neuronal transmitters, phosphorylation sites etc. by application of specific antibodies and visualization by fluorescence microscopy.
- Sample preparation for Western Blot by various extraction techniques.
- Separation of proteins by SDS-PAGE electrophoresis, followed by blotting onto nitrocellulose membrane.
- Chemiluminescent detection of specific proteins and/ or their phosphorylated form.
- DNA-isolation from laboratory mice tissue for molecular analysis.
- Identification and amplification of DNA fragments by PCR in order to verify gene expression in transgenic mice.