The Department of Neuronal Cell Biology is using molecular biology, cell biology, biochemistry and - in collaboration - electrophysiology and genetics.

Standard molecular biological techniques including cloning, PCR, real-time and qPCR, RNAi using pSuperior plasmids expressing shRNAs, Affymetrix microarrays (in collaboration with Martin Bilban, MUW), CLIP and deep sequencing (in collaboration with Jernej Ule, LMB Cambridge, UK),...

Standard cell biological techniques including rat hippocampal neurons in co-culture (Banker/Dotti culture system), transfection via calcium phosphate (Goetze et al., 2004, J. Neurobiol.), nucleofection using the Amaxa nucleofector (Amaxa, Köln, Germany; see Zeitelhofer et al., 2007, Nature Protocols), fluorescent microscopy of fixed or live hippocampal neurons, local perfusion, chemical activation of neurons, axon and dendritic outgrowth, dendritic spine morphogenesis,...

Standard biochemical techniques including brain extracts, differential centrifugation, density gradient centrifugation, analytical and preparative immunoprecipitations, SDS-PAGE and Western blotting, mass spectrometry (in collaboration with Giulio Superti-Furga and Keiryn Bennett (CeMM, Vienna).