- establishment and long-term cultures of CNS-antigen specific T lymphocyte lines
- establishment and culture of mixed glia, of astrocytes, microglia, and rat brain endothelial cells
- characterizations of cultured cells by flow cytometry, microarrays, real-time PCR and immunocytochemistry/confocal microscopy
- cytokine/chemokine profiling by ELISA and ELISPOT
- culture of glia, rat brain endothelial cells and neurons
- immunohistochemistry
- searching binding partners for pathogenic autoantibodies with phage display libraries
