Establishing and culturing cell lines stably expressing cloned membrane proteins
Flow cytometry
HPLC with electrochemical or fluorescence detection of neurotransmitters
Electrophysiology (patch-clamp)
Molecular Biology (site-directed mutagenesis, Western Blotting)
Stereotaxic injections
Receptor binding techniques
Slice autoradiography
Subregional dissection of the human brain