Generation of GABA_A receptor subunit-specific antibodies

Cell culture techniques (HEK cells, NT2 cells, SF9 cells)

Immunoaffinity chromatography, immunoprecipitation, Western blot, ELISA,

Immunocytochemistry, immunohistochemistry, confocal laser microscopy, FRET,

Biochemistry (subcellular fractionation, density gradient centrifugation, affinity chromatography)

Molecular Biology (cloning, sequencing, mutagenesis, recombinant receptors, chimeric receptors, fusion proteins, two hybrid system)

Receptor binding studies, binding pharmacology

Electrophysiology of GABAA receptors (Xenopus oocytes, HEK cells)