Generation of GABAA receptor subunit-specific antibodies
Cell culture techniques (HEK cells, NT2 cells, SF9 cells)
Immunoaffinity chromatography, immunoprecipitation, Western blot, ELISA,
Immunocytochemistry, immunohistochemistry, confocal laser microscopy, FRET,
Biochemistry (subcellular fractionation, density gradient centrifugation, affinity chromatography)
Molecular Biology (cloning, sequencing, mutagenesis, recombinant receptors, chimeric receptors, fusion proteins, two hybrid system)
Receptor binding studies, binding pharmacology
Electrophysiology of GABAA receptors (Xenopus oocytes, HEK cells)